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Wednesday, January 01, 2014 Matt D. Connecticut

Entry 23 of 189  
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Picture taken Dec. ’13 / Jan. ‘14

What I Want To See…

This shows a “normal” radical being formed in the media. While this is a trial seed, if you look closely at the top you will noticed a brown area which is the area of the endosperm/cotyledon leaves and this portion was underdeveloped and is actually just a thin skin of cells. However, in this case the media did the job of supplying not only the right signals to induce germination but also contains the food necessary for growth.

While this looks easy, I can assure you this is an accomplishment in itself. With micro propagation there are very thin margins of error. Also, the media is non-selective meaning that contamination is another factor that can complicate things. Basically a small dust particle in the air can cause an entire experiment to become a loss. To try and guard against this, all of these trials are carried out in a sterile laboratory setting to reduce the odds of contamination.
 



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