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Wednesday, January 01, 2014 Matt D. Connecticut

Entry 21 of 189  
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Picture taken Dec. ’13 / Jan. ‘14

Environmental Growth Chamber

I was fortunate to receive three of the 2328 Meier UOW DMG seeds. While I am honored to have any sent to myself, I can say that their quality was quite poor. When I did the drop test the seeds just about floated on the way down with my estimation of 25% of the seed full, and 75% being empty space.

As a result I knew normal germination methods would have an extremely low probability of success. I felt that there was basically no endosperm (food storage) in the seed, but it did feel like there may be an embryo in there but not much more. So, I went back to the embryo rescue research that I had done at the very beginning of this year. I was not able to run any trials but there were three different media recipes that I felt would provide increased odds for successful germination.

I was able to get media prepared that matched the specific recipes that were determined to have increased odds of radical formation so that an initial trial could be preformed. I then took different grades of seeds (meaning different estimated percentages of being solid) to run a trial to see if one media recipe worked better than another.
 



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