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Tuesday, November 11, 2014 MOpumpkins Springfield, Missouri

Entry 88 of 91
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This is embryo rescue after six days. Roots are developed and photosynthesis is starting to take place. Plantlets are now autotrophic, and can sustain themselves in sterile potting mix.
Why is this necessary? A fully developed endosperm normally contains starches, fats, vitamins, minerals and proteins that the germinating embryo breaks down into food. Embryo rescue is required when the endosperm is underdeveloped, and lacks the necessary components to allow germination. The MS media provides vitamins and minerals, and the sugar provides energy. Protein supplements are sometimes required, depending on the developmental stage of the embryo.

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